ORIGINAL ARTICLE   

Minerva Dental and Oral Science 2023 October;72(5):239-46

DOI: 10.23736/S2724-6329.23.04787-3

Copyright © 2023 EDIZIONI MINERVA MEDICA

lingua: Inglese

Comparison of salivary MicroRNA-6734, microRNA-3123 and microRNA-4483 expression in smoker and nonsmoker patients: a case control study

Parya ATAPOUR ¹, Abbas FARMANY ², Hamidreza ABDOLSAMADI ³, Ehsan HASHEMI ⁴, Mina JAZAERI ³ ✉

¹ Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Tabriz University of Medical Science, Tabriz, Iran; ² Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran; ³ Department of Oral and Maxillofacial Medicine, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran; ⁴ Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

BACKGROUND: Tobacco is a major risk factor in oral diseases. Considering the important role of expression miRNA molecules in different diseases, the present study aimed to compare the expression of salivary miRNA-6734, miRNA-3123 and miRNA-4483 in smoker and non-smoker peoples.
METHODS: In this case-control study, salivary samples were obtained from 30 smoker’s patients and 27 healthy nonsmokers and matched in term of age and sex. RNA was extracted in salivary samples and gene expression was evaluated in all samples. Statistical analysis of data was performed using T and chi-square tests by SPSS (Ver. 16) software at a significant level of less than 0.05.
RESULTS: The results of this study showed that the expression level of miRNA-3123 and miRNA-4483 in smokers group was 2.8 and 3.2, respectively, which was increased compared to non-smokers with expression level of 1 (P<0.01). There was a significant decrease in the expression rate of miRNA-6734 in smokers (0.6%) compared to non-smokers (P<0.05).
CONCLUSIONS: Based on the results of this study, considering the increasing miRNA-4483 and miRNA-3123 level, as well as the reducing miRNA-6734 level in smokers compared to non-smokers, it seems that evaluation of these two miRNAs as indicator for diagnosis and determination of prognosis of oral diseases associated with smoking can be used.

KEY WORDS: MicroRNAs; Saliva; Smoking; Cigarette smoking; RNA, untranslated